combined imaging of oxidative stress and microscopic

2020-7-31Thus the herein presented data combined with previous findings could be summarized in a proinflammatory vicious circle: AT 1 receptor activation by angiotensin II increases the production of IL-6 13–16 which in turn enhances AT 1 receptor expression leading to increased vasoconstriction enhanced oxidative stress inflammatory events Increased oxidative stress and disturbed mitochondrial morphology in NLRX1 KO mice during IRI Digital image analysis of immunohistochemistry for (A) 4-hydroxynonenal (4-HNE) and (B) nitrosylated tyrosine on kidney tissue sections from WT and KO animals (white and black bars respectively n = 8 per group) at the indicated time points during

Combined immunoelectron microscopic and

Read Combined immunoelectron microscopic and computer-assisted image analyses to detect advanced glycation end-products in human myocardium Histochemistry and Cell Biology on DeepDyve the largest online rental service for scholarly research with thousands of academic publications available at your fingertips

Oxidative stress (OS) has been recently implicated in the disease pathogenesis in inflammatory bowel disease (IBD) The aim of the study was to evaluate oxidative and antioxidative stress status and the risk of the atherosclerotic process in children with IBD and functional gastrointestinal disorders (FGID) The prospective study included a group of 71 children during a period of 2 years

2001-7-3Breath methylated alkane contour: a new marker of oxidative stress and disease United States Patent 6254547 Abstract: The alkane profile comprising the alveolar gradients of n-alkanes in breath having 2 to 20 carbons and the alveolar gradients of methylated C3-C20 alkanes is determined for the diagnosis of disease in mammals including

Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role of a vascular endothelial barrier in various pathological conditions Despite its importance as a marker of endothelial cell damage and impairment of the vascular system in vivo visualization of ESL has remained a challenging technical issue

2018-6-10confocal microscopic imaging technique and DCF-Da as a probe Noted that the generation of ROs reached plateau for merely 30 min and subsequently dissipated after receiving the drug for 3 h By using Mito-tracker red as a probe for labeling mitochondria we could vividly see that ROs generated was mainly concentrated around this organelle

MnSODtg Mice Control Myocardial Inflammatory and

The assembly and activation of NADPH oxidase result in an oxidative burst shown to be activated in response to T cruzi infection 18 Mice were infected by T cruzi and we investigated oxidative and inflammatory stress mitochondrial and tissue integrity and cardiac pathology Further we have used cutting‐edge 3‐dimensional imaging by

The imaging-based oxidative stress measurement in cyanobacteria using 2′ 7′-dichlorodihydrofluorescein diacetate (DCFH-DA) dye has the limitation of small sample size as the only selected number of cells are analyzed to measure the ROS levels

2018-7-23Oxidative stress is two sided: Whereas excessive oxidant challenge causes damage to biomolecules maintenance of a physiological level of oxidant challenge termed oxidative eustress is essential for governing life processes through redox signaling Recent interest has focused on the intricate ways by which redox signaling integrates these converse properties Redox balance is

2019-5-25The interest in graphene and its translation into commercial products has been expanding at a high pace Based on previously described pulmonary safety concerns for carbon nanomaterials there is a great need to define parameters guiding interactions between graphene-based materials and the pulmonary system The aim of the present study was to determine the importance of two critical

Oxidative damage arises in high light principally when the latter is in synergy with additional stress factors such as chilling temperatures or pollution Environmental stress can modify the photooxidative processes in various ways ranging from direct involvement in light‐induced free radical formation to the inhibition of metabolism that

First stress granules typically form during a variety of stress responses including responses to heat shock nutrient deprivation hypo-osmotic conditions and oxidative stress all of which inhibit bulk translation initiation leading to the release of most mRNAs from ribosomes (reviewed in Protter and Parker 2016) Subsets of these

Red blood cells (RBCs) are known to be the most suitable cells to study oxidative stress which is implicated in the etiopathology of many human diseases The goal of the current study was to develop a new effective approach for assessing oxidative stress in human living RBCs using two-photon microscopy To mimic oxidative stress in human living RBCs an in vitro model was generated followed

Read Combined immunoelectron microscopic and computer-assisted image analyses to detect advanced glycation end-products in human myocardium Histochemistry and Cell Biology on DeepDyve the largest online rental service for scholarly research with thousands of academic publications available at your fingertips

Dysregulation of Antioxidant Mechanisms Contributes

Dysregulation of Antioxidant Mechanisms Contributes to Increased Oxidative Stress in Calcific Aortic Valvular Stenosis in Humans Jordan D Miller Yi Chu Robert M Brooks Wayne E Richenbacher Ricardo Pea-Silva Donald D Heistad Oxidative stress plays an important role in development of atherosclerotic lesions We report that superoxide and H2O2 levels are increased in calcified and peri

The metabolome analysis (55 metabolites altered out of 673 detected p 0 05) confirmed lipotoxic conditions and oxidative stress by showing an activation of glutathione and ascorbate free radical scavenger systems Additionally we found metabolite alterations associated with hallmarks of hepatotoxicity such as γ-glutamyl dipeptides

2019-7-4The effects of a combination of the antioxidant astaxanthin (AX) and electrical stimulation (ES) on muscle mass and mitochondrial oxidative capacity were investigated in the soleus muscle of hindlimb unloaded rats Five groups of male Sprague-Dawley rats were used control 1-week hindlimb unloading (HU) HU + AX HU + ES and HU + AX + ES

2015-4-28Research project Study on non-invasive imaging technique of oxidative stress (Tohoku Institute of Technology)is a strategic research foundation grant-aided project for private universities suported by Ministry of Education Culture Sport Science and Technology (MEXT) Japan Development of novel technology for visualization of oxidative stress based on our original biophoton analysis

Autoluminescence imaging or biophoton imaging which captures the spontaneous ultra weak emission of photons typically associated with oxidative stress reactions (Havaux et al 2006) has been shown to discriminate the hypersensitive disease reaction (indicating plant resistance) from a lesion caused by attack of a virulent pathogen (Al-Daoude

Autoluminescence imaging or biophoton imaging which captures the spontaneous ultra weak emission of photons typically associated with oxidative stress reactions (Havaux et al 2006) has been shown to discriminate the hypersensitive disease reaction (indicating plant resistance) from a lesion caused by attack of a virulent pathogen (Al-Daoude

Oxidative Stress and Mitochondrial Activation as the Main Mechanisms Underlying Graphene Toxicity against Human Cancer Cells Anna Jarosz 1 Marta Skoda 1 Ilona Dudek 1 and Dariusz Szukiewicz 1 1 Department of General Experimental Pathology and Centre for Preclinical Research and Technology (CEPT) Medical University of Warsaw

OXIDATIVE STRESS Oxidative stress is a state in which either increased levels of cellular ROS are generated and/or cellular mechanisms to reduce the potentially damaging impact of ROS are of insufficient capacity [] This definition inherently dictates that as long as the antioxidant defense system is sufficiently capable of scavenging the generated reactive species increased ROS or reactive

A team led by researchers at I-Shou University School of Medicine tested the hypothesis that combined melatonin and exogenic adipose mesenchymal stem cell (ADMSC)-derived exosome treatment offers superior protection against liver ischemia-reperfusion (LIR) injury compared to either alone As CSE causes oxidative stress researchers from

That may explain our previous results in isolated nerve terminals showing that in the presence of an oxidative stress a concomitant elevation in [Na +] i acts deleteriously on in situ mitochondria [] The effect of K + loss from the cytoplasm is commonly ignored however it was shown that it can promote neuronal apoptosis [[258-260]]

OXIDATIVE STRESS Oxidative stress is a state in which either increased levels of cellular ROS are generated and/or cellular mechanisms to reduce the potentially damaging impact of ROS are of insufficient capacity [] This definition inherently dictates that as long as the antioxidant defense system is sufficiently capable of scavenging the generated reactive species increased ROS or reactive